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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD47 Monoclonal antibody, PBS Only

CD47 Monoclonal Antibody for IF/ICC, FC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4C4

Cat no : 60551-1-PBS

Synonyms

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CD47 Monoclonal antibody (60551-1-Ig)

    IF Staining of HepG2 using 60551-1-Ig (same clone as 60551-1-PBS)

    Immunofluorescent analysis of un-fixed HepG2 cells using CD47 antibody (60551-1-Ig, Clone: 1A4C4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60551-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using CD47 Monoclonal antibody (60551-1-Ig)

    FC experiment of human PBMCs using 60551-1-Ig (same clone as 60551-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 μg CD47 Monoclonal Antibody (60551-1-Ig, Clone: 1A4C4, right) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, left) was parallel stained as control. Cells were not fixed. This data was developed using the same antibody clone with 60551-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60551-1-PBS targets CD47 in IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD47 molecule
Calculated molecular weight 323 aa, 35 kDa
GenBank accession numberBC010016
Gene symbol CD47
Gene ID (NCBI) 961
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.