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  • Featured Product
  • KD/KO Validated

CD47 Monoclonal antibody, PBS Only

CD47 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1D8

Cat no : 66304-1-PBS

Synonyms

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD47 Monoclonal antibody (66304-1-Ig)

    WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CD47 Monoclonal antibody (66304-1-Ig)

    WB analysis of A549 using 66304-1-Ig (same clone as 66304-1-PBS)

    WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD47 Monoclonal antibody (66304-1-Ig)

    WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD47 Monoclonal antibody (66304-1-Ig)

    WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CD47 Monoclonal antibody (66304-1-Ig)

    IF Staining of A549 using 66304-1-Ig (same clone as 66304-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD47 antibody (66304-1-Ig, Clone: 1E1D8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66304-1-PBS targets CD47 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD47 fusion protein Ag14132 相同性解析による交差性が予測される生物種
Full Name CD47 molecule
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC010016
Gene symbol CD47
Gene ID (NCBI) 961
RRIDAB_2881687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.