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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD5 Polyclonal antibody, PBS Only

CD5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31502-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD5 Polyclonal antibody (31502-1-AP)

    WB analysis using 31502-1-AP (same clone as 31502-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31502-1-AP (CD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31502-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD5 Polyclonal antibody (31502-1-AP)

    IHC staining of human appendicitis using 31502-1-AP (same clone as 31502-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 31502-1-AP (CD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31502-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD5 Polyclonal antibody (31502-1-AP)

    IHC staining of human appendicitis using 31502-1-AP (same clone as 31502-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 31502-1-AP (CD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31502-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31502-1-PBS targets CD5 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD5 molecule
Calculated molecular weight55 kDa
Observed molecular weight60 kDa
GenBank accession numberNM_014207.4
Gene symbol CD5
Gene ID (NCBI) 921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).