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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD5 (UCHT2) Rabbit Recombinant Antibody, PBS Only

CD5 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHT2

Cat no : 65513-1-PBS

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD5 (UCHT2) Rabbit Recombinant Antibod (65513-1-PBS)

    FC experiment of human PBMCs using 65513-1-PBS

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD5 (UCHT2) Rabbit Recombinant Antibod (65513-1-PBS)

    FC experiment of human PBMCs using 65513-1-PBS

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD5 (UCHT2) Rabbit Recombinant Antibod (65513-1-PBS)

    FC experiment of human PBMCs using 65513-1-PBS

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000 (left and red), or stained with 0.2 ug Anti-Human CD5 Mouse Monoclonal Antibody (65152-1-Ig, Clone:UCHT2) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 (center and green). Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65513-1-PBS targets CD5 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD5 molecule
Calculated molecular weight 495 aa, 55 kDa
GenBank accession numberBC027901
Gene symbol CD5
Gene ID (NCBI) 921
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
FC protocol for CD5 antibody 65513-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols