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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD5 (UCHT2) Mouse IgG2a Recombinant Ant (65609-1-MR)

    FC experiment of human PBMCs using 65609-1-MR (same clone as 65609-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65609-1-MR, Clone: UCHT2) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65609-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD5 (UCHT2) Mouse IgG2a Recombinant Ant (65609-1-MR)

    FC experiment of human PBMCs using 65609-1-MR (same clone as 65609-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65609-1-MR, Clone:UCHT2) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65609-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD5 (UCHT2) Mouse IgG2a Recombinant Ant (65609-1-MR)

    FC experiment of human PBMCs using 65609-1-MR (same clone as 65609-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65609-1-MR, Clone:UCHT2) (left) or 0.25 ug Anti-Human CD5 (65152-1-Ig, Clone: UCHT2) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65609-1-PBS in a different storage buffer formulation.

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Anti-Human CD5 (UCHT2) Mouse IgG2a Recombinant Antibody, PBS Only

CD5 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65609-1-PBS
Clone No.UCHT2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

T-cell surface glycoprotein CD5, T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65609-1-PBS targets CD5 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD5 molecule
Calculated molecular weight 495 aa, 55 kDa
GenBank accession numberBC027901
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).