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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD5 Monoclonal antibody, PBS Only

CD5 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D12C10

Cat no : 67278-1-PBS

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IHC staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IHC staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IHC staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67278-1-Ig (CD5 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IF Staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (67278-1-Ig, Clone: 4D12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD5 Monoclonal antibody (67278-1-Ig)

    IF Staining of human tonsillitis using 67278-1-Ig (same clone as 67278-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (67278-1-Ig, Clone: 4D12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67278-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67278-1-PBS targets CD5 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD5 fusion protein Ag11065 相同性解析による交差性が予測される生物種
Full Name CD5 molecule
Calculated molecular weight 495 aa, 55 kDa
GenBank accession numberBC027901
Gene symbol CD5
Gene ID (NCBI) 921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).