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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD5 Recombinant antibody, PBS Only

CD5 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240244C2

Cat no : 83451-4-PBS

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of EL-4 cells using CD5 Recombinant antibody (83451-4-RR)

    WB analysis of EL-4 using 83451-4-RR (same clone as 83451-4-PBS)

    EL-4 cells were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using CD5 Recombinant antibody (83451-4-RR)

    WB analysis of mouse thymus using 83451-4-RR (same clone as 83451-4-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD5 Recombinant antibody (83451-4-RR)

    IHC staining of mouse spleen using 83451-4-RR (same clone as 83451-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD5 Recombinant antibody (83451-4-RR)

    IHC staining of mouse spleen using 83451-4-RR (same clone as 83451-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83451-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83451-4-RR

    Affinity and Kinetic Characterization of 83451-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83451-4-RR against Mouse CD5 were performed. The affinity constant is 89.9 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83451-4-PBS is CD5 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD5 antigen
Calculated molecular weight54 kDa
GenBank accession numberNM_007650.3
Gene symbol Cd5
Gene ID (NCBI) 12507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).