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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD5 Recombinant antibody

CD5 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240244C2

Cat no : 83451-4-RR

Synonyms

Cd5, CD5 antigen, Ly 1, Ly 12, Ly A, Lyt 1

Validation Data Gallery

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  • Western Blot (WB) analysis of EL-4 cells using CD5 Recombinant antibody (83451-4-RR)

    WB analysis of EL-4 using 83451-4-RR

    EL-4 cells were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CD5 Recombinant antibody (83451-4-RR)

    WB analysis of mouse thymus using 83451-4-RR

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inEL-4 cells, mouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83451-4-RR is CD5 Fusion Protein expressed in E. coli.

Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD5 antigen
Calculated molecular weight54 kDa
Observed molecular weight 55 kDa, 67 kDa
GenBank accession numberNM_007650.3
Gene symbol Cd5
Gene ID (NCBI) 12507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
WB protocol for CD5 antibody 83451-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols