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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD5 Recombinant antibody, PBS Only

CD5 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240428G2

Cat no : 83543-7-PBS

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD5 Recombinant antibody (83543-7-RR)

    WB analysis of Jurkat using 83543-7-RR (same clone as 83543-7-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 83543-7-RR (CD5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD5 Recombinant antibody (83543-7-RR)

    IHC staining of human tonsillitis using 83543-7-RR (same clone as 83543-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD5 Recombinant antibody (83543-7-RR)

    IHC staining of human tonsillitis using 83543-7-RR (same clone as 83543-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD5 Recombinant antibody (83543-7-RR)

    IHC staining of human ovary cancer using 83543-7-RR (same clone as 83543-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD5 Recombinant antibody (83543-7-RR)

    IHC staining of human ovary cancer using 83543-7-RR (same clone as 83543-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83543-7-RR

    Affinity and Kinetic Characterization of 83543-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83543-7-RR against Human CD5 were performed. The affinity constant is 2.09 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83543-7-PBS is CD5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD5 molecule
Calculated molecular weight55 kDa
GenBank accession numberNM_014207.4
Gene symbol CD5
Gene ID (NCBI) 921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.