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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD5 Rabbit Recombinant Antibody

CD5 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240428A12

Cat no : 98054-1-RR

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD5 Rabbit Recombinant Antibody (98054-1-RR)

    FC experiment of human PBMCs using 98054-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Rabbit Recombinant Antibody (98054-1-RR, Clone: 240428A12) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD5 Rabbit Recombinant Antibody (98054-1-RR)

    FC experiment of human PBMCs using 98054-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Rabbit Recombinant Antibody (98054-1-RR, Clone: 240428A12) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98054-1-RR

    Affinity and Kinetic Characterization of 98054-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98054-1-RR against Human CD5 were performed. The affinity constant is 0.27 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98054-1-RR is CD5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD5 molecule
Calculated molecular weight55 kDa
GenBank accession numberNM_014207.4
Gene symbol CD5
Gene ID (NCBI) 921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
FC protocol for CD5 antibody 98054-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols