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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody

CD5 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240244A7

Cat no : CL647-98038-2

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombina (CL647-98038-2)

    FC experiment of mouse splenocytes using CL647-98038-2

    1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3ε and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL647-98038-2, Clone:240244A7), or Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombina (CL647-98038-2)

    FC experiment of mouse splenocytes using CL647-98038-2

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL647-98038-2, Clone:240244A7) (red), or Isotype Control (blue). Cells were not fixed.

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Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98038-2 is CD5 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD5 antigen
Calculated molecular weight54 kDa
GenBank accession numberNM_007650.3
Gene symbol Cd5
Gene ID (NCBI) 12507
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD5 antibody CL647-98038-2Download protocol
Standard Protocols
Click here to view our Standard Protocols