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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using CD52 Monoclonal antibody (66784-1-Ig)

    WB analysis of human spleen using 66784-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66784-1-Ig (CD52 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CD52 Monoclonal antibody

CD52 Monoclonal Antibody for WB, ELISA
Cat No. 66784-1-Ig
Clone No.1B10E8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

Cambridge pathology 1 antigen, CAMPATH 1 antigen, CD52, CD52 molecule, CDW52, He5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66784-1-Ig targets CD52 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD52 fusion protein Ag17788 相同性解析による交差性が予測される生物種
Full Name CD52 molecule
Calculated molecular weight 6 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC000644
Gene Symbol CD52
Gene ID (NCBI) 1043
RRIDAB_2882129
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31358
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CD52 antibody 66784-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols