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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD53 Recombinant antibody (84837-1-RR)

    WB analysis using 84837-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84837-1-RR (CD53 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD53 Recombinant antibody (84837-1-RR)

    IHC staining of human tonsillitis using 84837-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84837-1-RR (CD53 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD53 Recombinant antibody (84837-1-RR)

    IHC staining of human tonsillitis using 84837-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84837-1-RR (CD53 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Daudi cells using CD53 Recombinant antibody (84837-1-RR)

    IF Staining of Daudi using 84837-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD53 Recombinant antibody (84837-1-RR)

    IF Staining of Jurkat using 84837-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84837-1-RR

    Affinity and Kinetic Characterization of 84837-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84837-1-RR against Human CD53 were performed. The affinity constant is below 1 pM.

CD53 Recombinant monoclonal antibody

CD53 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84837-1-RR
Clone No.242038C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242038C5, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin 25

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, Ramos cells, IM-9 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84837-1-RR targets CD53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2182

Product name: Recombinant Human CD53 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 107-181 aa of NM_000560.4

Sequence: EQKLNEYVAKGLTDSIHRYHSDNSTKAAWDSIQSFLQCCGINGTSDWTSGPPASCPSDRKVEGCYAKARLWFHSN

相同性解析による交差性が予測される生物種
Full Name CD53 molecule
Calculated molecular weight24kDa
Observed molecular weight 40 kDa
GenBank accession numberNM_000560.4
Gene Symbol CD53
Gene ID (NCBI) 963
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP19397
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).

Protocols

Product Specific Protocols
IF protocol for CD53 antibody 84837-1-RRDownload protocol
IHC protocol for CD53 antibody 84837-1-RRDownload protocol
WB protocol for CD53 antibody 84837-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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