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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombinant Antibody, PBS Only

ICAM-1/CD54 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65509-1-PBS

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombina (65509-1-PBS)

    FC experiment of human PBMCs using 65509-1-PBS

    1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-PBS, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombina (65509-1-RR)

    FC experiment of human PBMCs using 65509-1-RR (same clone as 65509-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65509-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombina (65509-1-RR)

    FC experiment of human PBMCs using 65509-1-RR (same clone as 65509-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65509-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombina (65509-1-PBS)

    FC experiment of human PBMCs using 65509-1-PBS

    1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-PBS, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombina (65509-1-PBS)

    FC experiment of human PBMCs using 65509-1-PBS

    1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-PBS, Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left and red), or stained with 0.05 ug Anti-Human CD54 Mouse Monoclonal Antibody (Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and green). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombina (65509-1-RR)

    FC experiment of human PBMCs using 65509-1-RR (same clone as 65509-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left and red), or stained with 0.05 ug Anti-Human CD54 Mouse Monoclonal Antibody (Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and green). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65509-1-PBS in a different storage buffer formulation.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65509-1-PBS targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
FC protocol for ICAM-1/CD54 antibody 65509-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols