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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human ICAM-1/CD54 (15.2), PBS Only

ICAM-1/CD54 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65075-1-PBS

Synonyms

CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50085-1

    Sandwich ELISA standard curve of MP50085-1

    Sandwich ELISA standard curve of MP50085-1, ICAM-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65075-1-PBS. Detection antibody: 68693-1-PBS. Standard: Eg0570. Range: 62.5-4000 pg/mL.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) (65075-1-Ig)

    FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) (65075-1-Ig)

    FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65075-1-PBS targets ICAM-1/CD54 as part of a matched antibody pair:

MP50085-1: 65075-1-PBS capture and 68693-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
RRIDAB_2918379
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).