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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD55 Recombinant antibody, PBS Only

CD55 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D2

Cat no : 82781-6-PBS

Synonyms

TC, DAF, CROM, CR, Complement decay-accelerating factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD55 Recombinant antibody (82781-6-RR)

    WB analysis using 82781-6-RR (same clone as 82781-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD55 Recombinant antibody (82781-6-RR)

    WB analysis using 82781-6-RR (same clone as 82781-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD55 Recombinant antibody (82781-6-RR)

    IHC staining of human placenta using 82781-6-RR (same clone as 82781-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD55 Recombinant antibody (82781-6-RR)

    IHC staining of human placenta using 82781-6-RR (same clone as 82781-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD55 Recombinant antibody (82781-6-RR)

    IHC staining of human lung cancer using 82781-6-RR (same clone as 82781-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD55 Recombinant antibody (82781-6-RR)

    IHC staining of human lung cancer using 82781-6-RR (same clone as 82781-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CD55 Recombinant antibody (82781-6-RR)

    IF Staining of A549 using 82781-6-RR (same clone as 82781-6-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD55 antibody (82781-6-RR, Clone: 2D2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82781-6-RR

    Affinity and Kinetic Characterization of 82781-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82781-6-RR against Human CD55 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82781-6-PBS targets CD55 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD55 fusion protein Ag25026 相同性解析による交差性が予測される生物種
Full Name CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Calculated molecular weight 41 kDa
Observed molecular weight75 kDa
GenBank accession numberBC001288
Gene symbol CD55
Gene ID (NCBI) 1604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).