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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse NCAM1/CD56 Rabbit Recombinant Antibody, PBS Only

NCAM1/CD56 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240936A4

Cat no : 98118-1-PBS

Synonyms

Ncam1, CD56, 240936A4, NCAM 1, Ncam

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse bone marrow cells using Anti-Mouse NCAM1/CD56 Rabbit Recombinant Antibody (98118-1-RR)

    FC experiment of mouse bone marrow cells using 98118-1-RR (same clone as 98118-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD56 Rabbit Recombinant Antibody (98118-1-RR, Clone: 240936A4) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98118-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse bone marrow cells using Anti-Mouse NCAM1/CD56 Rabbit Recombinant Antibody (98118-1-RR)

    FC experiment of mouse bone marrow cells using 98118-1-RR (same clone as 98118-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD56 Rabbit Recombinant Antibody (98118-1-RR, Clone:240936A4) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98118-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98118-1-RR

    Affinity and Kinetic Characterization of 98118-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98118-1-RR against Mouse CD56 were performed. The affinity constant is 9.50 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98118-1-PBS is NCAM1/CD56 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 1
Calculated molecular weight119 kDa
Gene symbol Ncam1
Gene ID (NCBI) 17967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56, NCAM, and MSK39) is a membrane-bound cell surface glycoprotein and a member of the immunoglobulin supergene family, which induces cell-to-cell interactions during embryonic development, cell migration, and organogenesis (PMID: 8013635; 1855476; 1391354). Mouse natural killer (NK) cells do not express the murine homolog of CD56 (PMID: 17100875). The molecular weight of NCAM1 changes from the 120 kDa isoform in normal tissue to the 140/180 kDa isoforms in tumors (PMID: 10086383).