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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated B3GAT1 Monoclonal antibody

B3GAT1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F4A11

Cat no : CL488-66711

Synonyms

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated B3GAT1 Monoclonal an (CL488-66711)

    FC experiment of K-562 using CL488-66711

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD57 (CL488-66711, Clone:1F4A11) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66711 targets B3GAT1 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen B3GAT1 fusion protein Ag13593 相同性解析による交差性が予測される生物種
Full Name beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa, 100 kDa
GenBank accession numberBC010466
Gene symbol B3GAT1
Gene ID (NCBI) 27087
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B3GAT1 is an enzyme encoded by the B3GAT1 gene, whose enzymatic activity generates the CD57 epitope on other cell surface proteins. In immunology, the CD57 antigen (CD stands for cluster of differentiation) is also known as HNK1 (human natural killer-1) or LEU7. B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 B3GAT1 antibody CL488-66711Download protocol
Standard Protocols
Click here to view our Standard Protocols