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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD59 Polyclonal antibody

CD59 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30223-1-AP

Synonyms

MAC-inhibitory protein, HRF-20, CD59 glycoprotein, CD59 antigen, CD 59

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD59 Polyclonal antibody (30223-1-AP)

    WB analysis using 30223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30223-1-AP (CD59 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD59 Polyclonal antibody (30223-1-AP)

    IHC staining of human placenta using 30223-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30223-1-AP (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD59 Polyclonal antibody (30223-1-AP)

    IHC staining of human tonsillitis using 30223-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30223-1-AP (CD59 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD59 Polyclonal antibody (30223-1-AP)

    IHC staining of human tonsillitis using 30223-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30223-1-AP (CD59 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD59 Polyclonal antibody (30223-1-AP)

    IHC staining of human placenta using 30223-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30223-1-AP (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CD59 Polyclonal antibody (30223-1-AP)

    IF Staining of BxPC-3 using 30223-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CD59 antibody (30223-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inBxPC-3 cells, HT-1080 cells, HT-29 cells, HUVEC cells, HeLa cells, U-87 MG cells
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30223-1-AP targets CD59 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD59 fusion protein Ag32557 相同性解析による交差性が予測される生物種
Full Name CD59 molecule, complement regulatory protein
Calculated molecular weight 19 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC001506
Gene symbol CD59
Gene ID (NCBI) 966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

Protocols

Product Specific Protocols
WB protocol for CD59 antibody 30223-1-APDownload protocol
IHC protocol for CD59 antibody 30223-1-APDownload protocol
IF protocol for CD59 antibody 30223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols