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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD59 Monoclonal antibody

CD59 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E3A1

Cat no : 68222-1-Ig

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD59 Monoclonal antibody (68222-1-Ig)

    WB analysis using 68222-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD59 Monoclonal antibody (68222-1-Ig)

    WB analysis using 68222-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IHC staining of human placenta using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IHC staining of human placenta using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IHC staining of human cervical cancer using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IHC staining of human cervical cancer using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IHC staining of human lung cancer using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IHC staining of human lung cancer using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IHC staining of human urothelial carcinoma using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IHC staining of human urothelial carcinoma using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IF Staining of human placenta using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IF Staining of human placenta using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IF Staining of human placenta using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD59 Monoclonal antibody (68222-1-Ig)

    IF Staining of human placenta using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CD59 Monoclonal antibody (68222-1-Ig)

    IF Staining of BxPC-3 using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman colostrum, A549 cells, HeLa cells, MCF-7 cells, HepG2 cells, K-562 cells, Jurkat cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, human lung cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68222-1-Ig targets CD59 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD59 fusion protein Ag32834 相同性解析による交差性が予測される生物種
Full Name CD59 molecule, complement regulatory protein
Calculated molecular weight 19 kDa
Observed molecular weight18-25 kDa
GenBank accession numberBC001506
Gene symbol CD59
Gene ID (NCBI) 966
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

Protocols

Product Specific Protocols
WB protocol for CD59 antibody 68222-1-IgDownload protocol
IHC protocol for CD59 antibody 68222-1-IgDownload protocol
IF protocol for CD59 antibody 68222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols