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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD59 Rabbit Recombinant Antibody, PBS Only

CD59 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241311A4

Cat no : 98099-1-PBS

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241311A4

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR)

    FC experiment of human PBMCs using 98099-1-RR (same clone as 98099-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG, Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98099-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR)

    FC experiment of human PBMCs using 98099-1-RR (same clone as 98099-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98099-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR)

    FC experiment of human PBMCs using 98099-1-RR (same clone as 98099-1-PBS)

    1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98099-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98099-1-RR

    Affinity and Kinetic Characterization of 98099-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98099-1-RR against Human CD59 were performed. The affinity constant is 0.888 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98099-1-PBS targets CD59 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD59 molecule, complement regulatory protein
Calculated molecular weight 19 kDa
GenBank accession numberBC001506
Gene symbol CD59
Gene ID (NCBI) 966
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.