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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD6 Polyclonal antibody (16763-1-AP)

    IHC staining of human tonsillitis using 16763-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16763-1-AP (CD6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD6 Polyclonal antibody (16763-1-AP)

    IHC staining of human tonsillitis using 16763-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16763-1-AP (CD6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD6 Polyclonal antibody (16763-1-AP)

    IHC staining of human tonsillitis using 16763-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16763-1-AP (CD6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD6 Polyclonal antibody (16763-1-AP)

    IHC staining of human tonsillitis using 16763-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16763-1-AP (CD6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD6 Polyclonal antibody

CD6 Polyclonal Antibody for IHC, ELISA
Cat No. 16763-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

TP120, T-cell differentiation antigen CD6, T12, Soluble CD6, CD6 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16763-1-AP targets CD6 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD6 fusion protein Ag10336 相同性解析による交差性が予測される生物種
Full Name CD6 molecule
Calculated molecular weight 668 aa, 72 kDa
GenBank accession numberBC078669
Gene Symbol CD6
Gene ID (NCBI) 923
RRIDAB_2878312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30203
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD6 is a type I transmembrane glycoprotein of 105-130 kDa that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.

Protocols

Product Specific Protocols
IHC protocol for CD6 antibody 16763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols