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  • Featured Product
  • KD/KO Validated

CD61 / Integrin beta 3 Monoclonal antibody, PBS Only

CD61 / Integrin beta 3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D8E5

Cat no : 66952-1-PBS

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using CD61 / Integrin beta 3 Monoclonal antibody (66952-1-Ig)

    WB analysis of HUVEC using 66952-1-Ig (same clone as 66952-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66952-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CD61 / Integrin beta 3 Monoclonal antibody (66952-1-Ig)

    WB analysis of human placenta using 66952-1-Ig (same clone as 66952-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66952-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD61 / Integrin beta 3 Monoclonal antibody (66952-1-Ig)

    IHC staining of human placenta using 66952-1-Ig (same clone as 66952-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66952-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD61 / Integrin beta 3 Monoclonal antibody (66952-1-Ig)

    IHC staining of human placenta using 66952-1-Ig (same clone as 66952-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66952-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66952-1-PBS targets CD61 / Integrin beta 3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD61 / Integrin beta 3 fusion protein Ag27834 相同性解析による交差性が予測される生物種
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Calculated molecular weight 788 aa, 87 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC127666
Gene symbol ITGB3
Gene ID (NCBI) 3690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.