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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood platelets using Anti-Human CD62P (AK4) Mouse IgG2a Recombinant Ant (65587-1-MR)

    FC experiment of human peripheral blood platelets using 65587-1-MR

    1x10^6 thrombin treated human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD62P Mouse IgG2a Recombinant Antibody (65587-1-MR, Clone: AK4) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Untreated human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD62P Mouse IgG2a Recombinant Antibody (65587-1-MR, Clone: AK4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L)(black). Cells were not fixed.

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Anti-Human CD62P (AK4) Mouse IgG2a Recombinant Antibody

CD62P Uni-rAbTM Recombinant Antibody for FC
Cat No. 65587-1-MR
Clone No.AK4

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Synonyms

CD62, CD62P, FLJ45155, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, SELP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inThrombin treated human peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65587-1-MR targets CD62P in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human platelets 相同性解析による交差性が予測される生物種
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Calculated molecular weight 830 aa, 91 kDa
GenBank accession numberBC028067
Gene Symbol CD62P
Gene ID (NCBI) 6403
RRIDAB_3670337
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Protocols

Product Specific Protocols
FC protocol for CD62P antibody 65587-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols