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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD63 Monoclonal antibody, PBS Only

CD63 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D4D1

Cat no : 67605-1-PBS

Synonyms

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen, CD 63

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD63 Monoclonal antibody (67605-1-Ig)

    WB analysis using 67605-1-Ig (same clone as 67605-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD63 Monoclonal antibody (67605-1-Ig)

    WB analysis using 67605-1-Ig (same clone as 67605-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IHC staining of human colon cancer using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IHC staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IHC staining of human colon cancer using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IHC staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IF Staining of human lymphoma using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IF Staining of human lymphoma using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human malignant melanoma tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IF Staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human malignant melanoma tissue using CD63 Monoclonal antibody (67605-1-Ig)

    IF Staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67605-1-PBS targets CD63 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD63 fusion protein Ag19690 相同性解析による交差性が予測される生物種
Full Name CD63 molecule
Calculated molecular weight 26 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002349
Gene symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.