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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD63 Recombinant antibody (84507-4-RR)

    WB analysis using 84507-4-RR (same clone as 84507-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84507-4-RR (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84507-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Recombinant antibody (84507-4-RR)

    IHC staining of human tonsillitis using 84507-4-RR (same clone as 84507-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84507-4-RR (CD63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84507-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Recombinant antibody (84507-4-RR)

    IHC staining of human tonsillitis using 84507-4-RR (same clone as 84507-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84507-4-RR (CD63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84507-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CD63 Recombinant antibody (84507-4-RR)

    IF Staining of A431 using 84507-4-RR (same clone as 84507-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD63 antibody (84507-4-RR, Clone: 241964H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84507-4-PBS in a different storage buffer formulation.

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CD63 Recombinant antibody, PBS Only

CD63 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84507-4-PBS
Clone No.241964H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen, CD 63

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84507-4-PBS targets CD63 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD63 molecule
Calculated molecular weight 26 kDa
Observed molecular weight30-60 kDa
GenBank accession numberBC002349
Gene Symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08962
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that regulate cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein translocated to the plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it is a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.