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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD68 Polyclonal antibody (30929-1-AP)

    WB analysis using 30929-1-AP (same clone as 30929-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30929-1-AP (CD68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD68 Polyclonal antibody (30929-1-AP)

    IHC staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD68 Polyclonal antibody (30929-1-AP)

    IHC staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD68 Polyclonal antibody (30929-1-AP)

    IHC staining of mouse liver using 30929-1-AP (same clone as 30929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD68 Polyclonal antibody (30929-1-AP)

    IHC staining of mouse liver using 30929-1-AP (same clone as 30929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD68 Polyclonal antibody (30929-1-AP)

    IF Staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD68 Polyclonal antibody (30929-1-AP)

    IF Staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

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Mouse CD68 Polyclonal antibody, PBS Only

CD68 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 30929-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30929-1-PBS targets CD68 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD68 antigen
Calculated molecular weight35 kDa
GenBank accession numberNM_001291058.1
Gene Symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31996-1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.