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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD68 (KP1)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

KP1

Cat no : 65202-1-Ig

Synonyms

CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) (65202-1-Ig)

    FC experiment of human PBMCs using 65202-1-Ig

    1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65202-1-Ig, Clone:KP1) and FITC-Donkey anti-Mouse IgG at dilution 1:1000 (red), or Mouse IgG1 Isotype Control and FITC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) (65202-1-Ig)

    FC experiment of human PBMCs using 65202-1-Ig

    1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65202-1-Ig, Clone:KP1) and FITC-Donkey anti-Mouse IgG at dilution 1:1000, or Mouse IgG1 Isotype Control and FITC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Tested Applications

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65202-1-Ig targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Lysosomal fraction of human alveolar macrophages 相同性解析による交差性が予測される生物種
Full Name CD68 molecule
Calculated molecular weight 37 kDa
GenBank accession numberBC015557
Gene symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
FC protocol for CD68 antibody 65202-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols