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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) Rabbit IgG Recombinant Anti (65515-1-RR)

    FC experiment of human PBMCs using 65515-1-RR

    1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) Rabbit IgG Recombinant Anti (65515-1-RR)

    FC experiment of human PBMCs using 65515-1-RR

    1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) Rabbit IgG Recombinant Anti (65515-1-RR)

    FC experiment of human PBMCs using 65515-1-RR

    1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) (red) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.1 ug Anti-Human CD68 Mouse Monoclonal Antibody (Clone:KP1) (65202-1-Ig in green; or from another vendor in orange) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.

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Anti-Human CD68 (KP1) Rabbit IgG Recombinant Antibody

CD68 Recombinant Antibody for FC (Intra)
Cat No. 65515-1-RR
Clone No.KP1
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra)

Synonyms

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65515-1-RR targets CD68 in WB, IHC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Lysosomal fraction of human alveolar macrophages 相同性解析による交差性が予測される生物種
Full Name CD68 molecule
Calculated molecular weight 37 kDa
GenBank accession numberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
RRIDAB_3670302
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP34810
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
FC protocol for CD68 antibody 65515-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Gene

Potential predictive value of immune-related genes FUCA1 and NCKAP1L for osteosarcoma metastasis

Authors - Xuan Wang
View Article