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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) Mouse IgG1 Recombinant Antib (65521-1-PBS)

    FC experiment of human PBMCs using 65521-1-PBS

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 (65521-1-PBS, Clone: KP1) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) Mouse IgG1 Recombinant Antib (65521-1-MR)

    FC experiment of human PBMCs using 65521-1-MR (same clone as 65521-1-PBS)

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 (65521-1-MR, Clone:KP1) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were not fixed. This data was developed using the same antibody clone with 65521-1-PBS in a different storage buffer formulation.

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Anti-Human CD68 (KP1) Mouse IgG1 Recombinant Antibody, PBS Only

CD68 Recombinant Antibody for FC, FC (Intra)
Cat No. 65521-1-PBS
Clone No.KP1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC, FC (Intra)

Synonyms

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65521-1-PBS targets CD68 in FC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Immunogen Lysosomal fraction of human alveolar macrophages 相同性解析による交差性が予測される生物種
Full Name CD68 molecule
Calculated molecular weight 37 kDa
GenBank accession numberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP34810
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
FC protocol for CD68 antibody 65521-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols