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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Anti (65593-1-MR)

    FC experiment of human PBMCs using 65593-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone: KP1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Anti (65593-1-MR)

    FC experiment of human PBMCs using 65593-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Anti (65593-1-MR)

    FC experiment of human PBMCs using 65593-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (left) or 0.25 ug Anti-Human CD68 (65202-1-Ig, Clone: KP1) (right) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody

CD68 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 65593-1-MR
Clone No.KP1

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Synonyms

CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65593-1-MR targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD68 molecule
Calculated molecular weight 37 kDa
GenBank accession numberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
RRIDAB_3670342
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
FC protocol for CD68 antibody 65593-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols