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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD68 Recombinant antibody (84596-4-RR)

    WB analysis using 84596-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84596-4-RR (CD68 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD68 Recombinant antibody (84596-4-RR)

    IHC staining of human tonsillitis using 84596-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD68 Recombinant antibody (84596-4-RR)

    IHC staining of human tonsillitis using 84596-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD68 Recombinant antibody (84596-4-RR)

    IHC staining of mouse spleen using 84596-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD68 Recombinant antibody (84596-4-RR)

    IHC staining of mouse spleen using 84596-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD68 Recombinant antibody

CD68 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84596-4-RR
Clone No.241810B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SCARD1, Macrosialin, GP110, CD68 molecule, 241810B3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJ774A.1 cells, U-937 cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84596-4-RR targets CD68 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD68 molecule
Calculated molecular weight 37 kDa
Observed molecular weight80-90 kDa
GenBank accession numberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP34810
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in the phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
WB protocol for CD68 antibody 84596-4-RRDownload protocol
IHC protocol for CD68 antibody 84596-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols