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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse CD68 Rabbit Recombinant Antibody, PBS Only

CD68 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230504G5

Cat no : 98029-1-PBS

Synonyms

Scard1, gp110, CD68 antigen, 230504G5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR)

    FC experiment of mouse peritoneal macrophages using 98029-1-RR (same clone as 98029-1-PBS)

    1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR, Clone: 230504G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Mouse CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98029-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR)

    FC experiment of mouse peritoneal macrophages using 98029-1-RR (same clone as 98029-1-PBS)

    1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR, Clone: 230504G5) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98029-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98029-1-RR

    Affinity and Kinetic Characterization of 98029-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98029-1-RR against Mouse CD68 were performed. The affinity constant is 10.8 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98029-1-PBS is CD68 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD68 antigen
Calculated molecular weight35 kDa
GenBank accession numberNM_001291058.1
Gene symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.