CD69 Monoclonal antibody
CD69 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG3
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3D10A2
Cat no : 68827-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-937 cells, THP-1 cells, HL-60 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68827-1-Ig targets CD69 in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG3 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD69 fusion protein Ag30963 相同性解析による交差性が予測される生物種 |
Full Name | CD69 molecule |
Calculated molecular weight | 23 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC007037 |
Gene symbol | CD69 |
Gene ID (NCBI) | 969 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, and several subsets of tissue-resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in regulating immune responses (PMID: 15745855).
Protocols
Product Specific Protocols | |
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WB protocol for CD69 antibody 68827-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |