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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD69 Recombinant antibody (84258-7-RR)

    WB analysis using 84258-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84258-7-RR (CD69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84258-7-RR

    Affinity and Kinetic Characterization of 84258-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84258-7-RR against Human CD69 were performed. The affinity constant is 0.776 nM.

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CD69 Recombinant antibody

CD69 Recombinant Antibody for WB, ELISA
Cat No. 84258-7-RR
Clone No.241552A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84258-7-RR targets CD69 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD69 molecule
Calculated molecular weight23 kDa
Observed molecular weight35 kDa
GenBank accession numberNM_001781.2
Gene Symbol CD69
Gene ID (NCBI) 969
RRIDAB_3671805
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07108
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

Protocols

Product Specific Protocols
WB protocol for CD69 antibody 84258-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols