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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD7 Monoclonal antibody, PBS Only

CD7 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10D8

Cat no : 60209-1-PBS

Synonyms

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD7 Monoclonal antibody (60209-1-Ig)

    WB analysis of Jurkat using 60209-1-Ig (same clone as 60209-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-1-Ig (CD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human appendicitis using 60209-1-Ig (same clone as 60209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human appendicitis using 60209-1-Ig (same clone as 60209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human tonsillitis using 60209-1-Ig (same clone as 60209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human lymphoma using 60209-1-Ig (same clone as 60209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-1-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human lymphoma using 60209-1-Ig (same clone as 60209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-1-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human tonsillitis using 60209-1-Ig (same clone as 60209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IF Staining of human tonsillitis using 60209-1-Ig (same clone as 60209-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IF Staining of human tonsillitis using 60209-1-Ig (same clone as 60209-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60209-1-PBS targets CD7 in IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD7 fusion protein Ag1805 相同性解析による交差性が予測される生物種
Full Name CD7 molecule
Calculated molecular weight 240 aa, 25 kDa
GenBank accession numberBC009293
Gene symbol CD7
Gene ID (NCBI) 924
RRIDAB_10953398
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.