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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD70 Monoclonal antibody, PBS Only

CD70 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11A10

Cat no : 67749-1-PBS

Synonyms

CD27 L, CD27 ligand, CD27L, CD27LG, CD70, CD70 antigen, CD70 molecule, TNFSF7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD70 Monoclonal antibody (67749-1-Ig)

    WB analysis using 67749-1-Ig (same clone as 67749-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67749-1-Ig (CD70 antibody) at dilution of 1:1000, 2000, 4000 and 8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD70 Monoclonal antibody (67749-1-Ig)

    IHC staining of human tonsillitis using 67749-1-Ig (same clone as 67749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67749-1-Ig (CD70 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD70 Monoclonal antibody (67749-1-Ig)

    IHC staining of human tonsillitis using 67749-1-Ig (same clone as 67749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67749-1-Ig (CD70 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67749-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD70 Monoclonal antibody (67749-1-Ig)

    IF Staining of human tonsillitis using 67749-1-Ig (same clone as 67749-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD70 antibody (67749-1-Ig, Clone: 1A11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67749-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67749-1-PBS targets CD70 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD70 fusion protein Ag28070 相同性解析による交差性が予測される生物種
Full Name CD70 molecule
Calculated molecular weight 21 kDa
GenBank accession numberBC000725
Gene symbol CD70
Gene ID (NCBI) 970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.