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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD70 Monoclonal antibody (67749-1-Ig)

    WB analysis using 67749-1-Ig (same clone as 67749-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67749-1-Ig (CD70 antibody) at dilution of 1:1000, 2000, 4000 and 8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD70 Monoclonal antibody (67749-1-Ig)

    IHC staining of human tonsillitis using 67749-1-Ig (same clone as 67749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67749-1-Ig (CD70 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD70 Monoclonal antibody (67749-1-Ig)

    IHC staining of human tonsillitis using 67749-1-Ig (same clone as 67749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67749-1-Ig (CD70 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67749-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD70 Monoclonal antibody (67749-1-Ig)

    IF Staining of human tonsillitis using 67749-1-Ig (same clone as 67749-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD70 antibody (67749-1-Ig, Clone: 1A11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67749-1-PBS in a different storage buffer formulation.

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CD70 Monoclonal antibody, PBS Only

CD70 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67749-1-PBS
Clone No.1A11A10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD27 L, CD27 ligand, CD27L, CD27LG, CD70, CD70 antigen, CD70 molecule, TNFSF7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67749-1-PBS targets CD70 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD70 fusion protein Ag28070 相同性解析による交差性が予測される生物種
Full Name CD70 molecule
Calculated molecular weight 21 kDa
GenBank accession numberBC000725
Gene Symbol CD70
Gene ID (NCBI) 970
RRIDAB_2918518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32970
Storage Buffer PBS only
Storage ConditionsStore at -80°C.