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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD70 Recombinant antibody (84455-2-RR)

    IF Staining of THP-1 using 84455-2-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD70 antibody (84455-2-RR, Clone: 241324B12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84455-2-RR

    Affinity and Kinetic Characterization of 84455-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84455-2-RR against Human CD70 were performed. The affinity constant is 67.0 pM.

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CD70 Recombinant antibody

CD70 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84455-2-RR
Clone No.241324B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

CD27LG, CD27-L, CD27L, CD27 ligand, CD27 L

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84455-2-RR targets CD70 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD70 molecule
Calculated molecular weight 21 kDa
GenBank accession numberBC000725
Gene Symbol CD70
Gene ID (NCBI) 970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CD70 antibody 84455-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols