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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD71 Polyclonal antibody

CD71 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30574-1-AP

Synonyms

TFRC, TFR1, TFR, T9, sTfR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD71 Polyclonal antibody (30574-1-AP)

    WB analysis using 30574-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30574-1-AP (CD71 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CD71 Polyclonal antibody (30574-1-AP)

    IP experiment of HeLa using 30574-1-AP

    IP result of anti-CD71 (IP:30574-1-AP, 4ug; Detection:30574-1-AP 1:10000) with HeLa cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Polyclonal antibody (30574-1-AP)

    IHC staining of human placenta using 30574-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30574-1-AP (CD71 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Polyclonal antibody (30574-1-AP)

    IHC staining of human placenta using 30574-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30574-1-AP (CD71 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD71 Polyclonal antibody (30574-1-AP)

    IF Staining of HeLa using 30574-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD71 antibody (30574-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CD71 Polyclonal antibody (30574-1-AP)

    IF Staining of MCF-7 using 30574-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (30574-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30574-1-AP targets CD71 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight85 kDa
Observed molecular weight80-90 kDa
Gene symbol CD71
Gene ID (NCBI) 7037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
WB protocol for CD71 antibody 30574-1-APDownload protocol
IHC protocol for CD71 antibody 30574-1-APDownload protocol
IF protocol for CD71 antibody 30574-1-APDownload protocol
IP protocol for CD71 antibody 30574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols