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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD71 Monoclonal antibody

CD71 Monoclonal Antibody for WB, IHC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, mouse

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

H68.4

Cat no : 65236-1-Ig

Synonyms

TFR1, TFR, T9, sTfR, p90

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD71 Monoclonal antibody (65236-1-Ig)

    WB analysis using 65236-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using CD71 Monoclonal antibody (65236-1-Ig)

    WB analysis of RAW 264.7 using 65236-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Monoclonal antibody (65236-1-Ig)

    IHC staining of human placenta using 65236-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Monoclonal antibody (65236-1-Ig)

    IHC staining of human placenta using 65236-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-3 cells, RAW 264.7 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65236-1-Ig targets CD71 in WB, IHC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
Observed molecular weight90 kDa
GenBank accession numberBC001188
Gene symbol CD71
Gene ID (NCBI) 7037
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
WB protocol for CD71 antibody 65236-1-IgDownload protocol
IHC protocol for CD71 antibody 65236-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols