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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD71 (OKT9) Rabbit IgG Recombinant Anti (65507-1-PBS)

    FC experiment of human PBMCs using 65507-1-PBS

    1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD71 (OKT9) Rabbit IgG Recombinant Anti (65507-1-RR)

    FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)

    1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD71 (OKT9) Rabbit IgG Recombinant Anti (65507-1-RR)

    FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)

    1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD71 (OKT9) Rabbit IgG Recombinant Anti (65507-1-PBS)

    FC experiment of human PBMCs using 65507-1-PBS

    1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD71 (OKT9) Rabbit IgG Recombinant Anti (65507-1-PBS)

    FC experiment of human PBMCs using 65507-1-PBS

    1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD71 (OKT9) Rabbit IgG Recombinant Anti (65507-1-RR)

    FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)

    1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.

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Anti-Human CD71 (OKT9) Rabbit IgG Recombinant Antibody, PBS Only

CD71 Recombinant Antibody for FC
Cat No. 65507-1-PBS
Clone No.OKT9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TFR1, TFR, T9, sTfR, p90

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65507-1-PBS targets CD71 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Human T-cell acute lymphoblastic leukemia cells 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
GenBank accession numberBC001188
Gene Symbol CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP02786
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for CD71 antibody 65507-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols