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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD71 Monoclonal antibody (66180-1-Ig)

    WB analysis using 66180-1-Ig (same clone as 66180-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CD71 Monoclonal antibody (66180-1-Ig)

    WB analysis of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

    WB result of CD71 antibody (66180-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD71 transfected HeLa cells. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CD71 Monoclonal antibody (66180-1-Ig)

    WB analysis of Raji using 66180-1-Ig (same clone as 66180-1-PBS)

    Untreated and PNGase F-treated lysates of Raji cells and HeLa cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of TF-1 cells using CD71 Monoclonal antibody (66180-1-Ig)

    WB analysis of TF-1 using 66180-1-Ig (same clone as 66180-1-PBS)

    TF-1 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CD71 Monoclonal antibody (66180-1-Ig)

    WB analysis of A549 using 66180-1-Ig (same clone as 66180-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using CD71 Monoclonal antibody (66180-1-Ig)

    IP experiment of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

    IP result of anti-CD71 (IP:66180-1-Ig, 5ug; Detection:66180-1-Ig 1:1000) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Monoclonal antibody (66180-1-Ig)

    IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Monoclonal antibody (66180-1-Ig)

    IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Monoclonal antibody (66180-1-Ig)

    IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD71 Monoclonal antibody (66180-1-Ig)

    IHC staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD71 Monoclonal antibody (66180-1-Ig)

    IHC staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CD71 Monoclonal antibody (66180-1-Ig)

    IF Staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CD71 Monoclonal antibody (66180-1-Ig)

    IF Staining of MCF-7 using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CD71 Monoclonal antibody (66180-1-Ig)

    IF Staining of K-562 using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CD71 Monoclonal antibody (66180-1-Ig)

    IF Staining of K-562 using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CD71 Monoclonal antibody, PBS Only

CD71 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 66180-1-PBS
Clone No.3C11F11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

TFR1, TFR, T9, sTfR, p90

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66180-1-PBS targets CD71 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD71 fusion protein Ag21612 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC001188
Gene Symbol CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
RRIDAB_2881575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02786
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.