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  • Featured Product
  • KD/KO Validated

CD71 Monoclonal antibody, PBS Only

CD71 Monoclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C11F11

Cat no : 66180-1-PBS

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

Validation Data Gallery

Applications:
  • WB analysis of Raji using 66180-1-Ig (same clone as 66180-1-PBS)

    WB analysis of Raji using 66180-1-Ig (same clone as 66180-1-PBS)

    Untreated and PNGase F-treated lysates of Raji cells and HeLa cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • WB analysis using 66180-1-Ig (same clone as 66180-1-PBS)

    WB analysis using 66180-1-Ig (same clone as 66180-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • WB analysis of TF-1 using 66180-1-Ig (same clone as 66180-1-PBS)

    WB analysis of TF-1 using 66180-1-Ig (same clone as 66180-1-PBS)

    TF-1 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66180-1-Ig (same clone as 66180-1-PBS)

    WB analysis of A549 using 66180-1-Ig (same clone as 66180-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

    IP experiment of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

    IP result of anti-CD71 (IP:66180-1-Ig, 5ug; Detection:66180-1-Ig 1:1000) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IHC staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    IHC staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IHC staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    IHC staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD71 antibody included
  • IF Staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    IF Staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66180-1-Ig (same clone as 66180-1-PBS)

    IF Staining of MCF-7 using 66180-1-Ig (same clone as 66180-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

  • FC experiment of Raji using 66180-1-Ig (same clone as 66180-1-PBS)

    FC experiment of Raji using 66180-1-Ig (same clone as 66180-1-PBS)

    1X10^6 Raji cells were stained with 0.2ug CD71 antibody (66180-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IgG with dilution 1:100. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66180-1-PBS targets CD71 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD71 fusion protein Ag21612 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC001188
Gene symbol TFRC
Gene ID (NCBI) 7037
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.