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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using CD72 Polyclonal antibody (13469-1-AP)

    WB analysis of mouse testis using 13469-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13469-1-AP (CD72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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CD72 Polyclonal antibody

CD72 Polyclonal Antibody for WB, ELISA
Cat No. 13469-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Lyb-2, LYB2, Lyb 2, CD72b, CD72 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13469-1-AP targets CD72 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD72 fusion protein Ag4262 相同性解析による交差性が予測される生物種
Full Name CD72 molecule
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
RRIDAB_10638306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

Protocols

Product Specific Protocols
WB protocol for CD72 antibody 13469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols