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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using Anti-Human CD72 (3F3) (65157-1-Ig)

    FC experiment of human peripheral blood lymphocytes using 65157-1-Ig

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD72 (65157-1-Ig, Clone: 3F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Anti-Human CD72 (3F3)

CD72 Monoclonal Antibody for FC
Cat No. 65157-1-Ig
Clone No.3F3

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Synonyms

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65157-1-Ig targets CD72 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Normal human lymphocytes from a lymph node 相同性解析による交差性が予測される生物種
Full Name CD72 molecule
GenBank accession numberBC011734
Gene Symbol CD72
Gene ID (NCBI) 971
RRIDAB_2918439
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by precipitation and chromatography
UNIPROT IDP21854
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

Protocols

Product Specific Protocols
FC protocol for CD72 antibody 65157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols