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Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using CD72 Monoclonal antibody (67424-1-Ig)

    WB analysis of Daudi using 67424-1-Ig (same clone as 67424-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 67424-1-Ig (CD72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD72 Monoclonal antibody (67424-1-Ig)

    IHC staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD72 Monoclonal antibody (67424-1-Ig)

    IHC staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD72 Monoclonal antibody (67424-1-Ig)

    IHC staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD72 Monoclonal antibody (67424-1-Ig)

    IF Staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD72 antibody (67424-1-Ig, Clone: 2C1C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using CD72 Monoclonal antibody (67424-1-Ig)

    IF Staining of Ramos using 67424-1-Ig (same clone as 67424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD72 antibody (67424-1-Ig, Clone: 2C1C6 ) at dilution of 1:1600 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

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CD72 Monoclonal antibody, PBS Only

CD72 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67424-1-PBS
Clone No.2C1C6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67424-1-PBS targets CD72 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD72 fusion protein Ag14405 相同性解析による交差性が予測される生物種
Full Name CD72 molecule
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
RRIDAB_2882663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21854
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).