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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD72 Recombinant antibody

CD72 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241301B3

Cat no : 84270-5-RR

Synonyms

LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD72 Recombinant antibody (84270-5-RR)

    WB analysis of Raji using 84270-5-RR

    Raji cells were subjected to SDS PAGE followed by western blot with 84270-5-RR (CD72 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84270-5-RR

    Affinity and Kinetic Characterization of 84270-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84270-5-RR against Human CD72 were performed. The affinity constant is 59.3 pM.

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Tested Applications

Positive WB detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84270-5-RR targets CD72 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD72 molecule
Calculated molecular weight 359 aa, 40 kDa
GenBank accession numberBC030227
Gene symbol CD72
Gene ID (NCBI) 971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

Protocols

Product Specific Protocols
WB protocol for CD72 antibody 84270-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols