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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD73 Polyclonal antibody

CD73 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 32131-1-AP

Synonyms

IMP-specific 5'-nucleotidase, Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD73 Polyclonal antibody (32131-1-AP)

    WB analysis using 32131-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32131-1-AP (CD73 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32131-1-AP targets CD73 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name 5' nucleotidase, ecto
Calculated molecular weight64kDa
Observed molecular weight65-70 kDa
GenBank accession numberNM_011851.4
Gene symbol Nt5e
Gene ID (NCBI) 23959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
WB protocol for CD73 antibody 32131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols