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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD73 (AD2) Mouse IgG1 Recombinant Antib (65531-1-MR)

    FC experiment of human PBMCs using 65531-1-MR (same clone as 65531-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 (65531-1-MR, Clone:AD2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed. This data was developed using the same antibody clone with 65531-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD73 (AD2) Mouse IgG1 Recombinant Antib (65531-1-PBS)

    FC experiment of human PBMCs using 65531-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 (65531-1-PBS, Clone: AD2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.25 ug Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (blue). Cells were not fixed.

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Anti-Human CD73 (AD2) Mouse IgG1 Recombinant Antibody, PBS Only

CD73 Recombinant Antibody for FC
Cat No. 65531-1-PBS
Clone No.AD2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Synonyms

NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65531-1-PBS targets CD73 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase, ecto (CD73)
Calculated molecular weight 29 kDa, 63 kDa
GenBank accession numberBC015940
Gene Symbol CD73
Gene ID (NCBI) 4907
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
FC protocol for CD73 antibody 65531-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols