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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Anti (65564-1-MR)

    FC experiment of human PBMCs using 65564-1-MR (same clone as 65564-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 Mouse Recombinant Antibody (65564-1-MR, Clone:AD2) or 0.5 ug Mouse IgG2a Isotype Contro (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65564-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Anti (65564-1-PBS)

    FC experiment of human PBMCs using 65564-1-PBS

    1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 Mouse Recombinant Antibody, PBS Only (65564-1-PBS, Clone:AD2) or 0.5 ug Mouse IgG2a Isotype Contro (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Anti (65564-1-MR)

    FC experiment of human PBMCs using 65564-1-MR (same clone as 65564-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 Mouse Recombinant Antibody (65564-1-MR, Clone: AD2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)(right), or 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone: AD2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)(left). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65564-1-PBS in a different storage buffer formulation.

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Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Antibody, PBS Only

CD73 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65564-1-PBS
Clone No.AD2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73)

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65564-1-PBS targets CD73 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Pre-B leukemia cell line 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase, ecto (CD73)
Calculated molecular weight 29 kDa, 63 kDa
GenBank accession numberBC015940
Gene Symbol CD73
Gene ID (NCBI) 4907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21589
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
FC protocol for CD73 antibody 65564-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols