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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD79a Recombinant antibody

CD79a Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1I17

Cat no : 82747-2-RR

Synonyms

MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD79a Recombinant antibody (82747-2-RR)

    IF Staining of Raji using 82747-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD79A antibody (82747-2-RR, Clone: 1I17 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82747-2-RR

    Affinity and Kinetic Characterization of 82747-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82747-2-RR against Human CD79A were performed. The affinity constant is 8.32 nM.

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Tested Applications

Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82747-2-RR targets CD79a in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD79a fusion protein Ag17924 相同性解析による交差性が予測される生物種
Full Name CD79a molecule, immunoglobulin-associated alpha
Calculated molecular weight 226 aa, 25 kDa
GenBank accession numberBC113733
Gene symbol CD79A
Gene ID (NCBI) 973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

Protocols

Product Specific Protocols
IF protocol for CD79a antibody 82747-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols